2.1.3 The physicochemical and basic biological characteristics
of the W541vaccine protein
The
physicochemical properties of the vaccine proteins, including amino acid
composition, molecular weight, theoretical isoelectric point (pI),
instability index (II), aliphatic index (AI), and grand average of
hydropathicity (GRAVY), were predicted using the ProtParam
server(39).
The SignaIP 4.1 server (40), MHMM 2.0 server, and Cell-PLoc 2.0
server(41) were used to predict the presence of signal peptide
sequences, transmembrane regions, and subcellular localization of the
vaccine proteins after expression within host cells, respectively. The
antigenicity, allergenicity, and toxicity of the vaccine proteins were
predicted using the VaxiJen server(42), AllerTop server(39), and
ToxinPred server(43), respectively. Human homology analysis on the
vaccine proteins was performed using the BLAST server(44).